Amino Acid Analysis Service

gutfloraomics

Amino Acid Analysis Service

Amino acids are the basic components of biologically functional macromolecular proteins, and are the basic substances that constitute proteins required for animal nutrition.

Protein in food can be broken down by intestinal bacteria to produce peptides and amino acids. Among them, branched chain amino acids such as valine, leucine and isoleucine can be further metabolized by bacteria to form branched chain fatty acids. The bacterial metabolites of aromatic amino acids such as phenylalanine, tryptophan and tyrosine are phenols and indole substances.

Amino acids play an important role in material metabolism and immune function regulation. The full spectrum of amino acids is a balanced state in the body. The imbalance of this state is the cause or manifestation of many diseases. Amino acids are involved in metabolism, tumors, immunity, cardiovascular and cerebrovascular diseases, nervous system, kidney disease, diabetes, sub-health, senile diseases and many other links such as human growth and development, nutrition and health, musculoskeletal growth, hormone secretion, detoxification and so on.

Metabolic pathways utilized for production of amino acids and amino acid-related compounds in microorganisms

Metabolic pathways utilized for production of amino acids and amino acid-related compounds in microorganisms (Hirasawa et al., 2016)

Creative Proteomics uses the LC-MS/MS technology platform to achieve absolute quantification of amino acids in a variety of species and biological samples to meet different detection requirements. In addition, we can also implement one-stop, personalized data analysis to help you achieve better data interpretation.

What We Can Provide

Below is a partial list of amino acids that we can detect. If you want to detect other metabolites that are not in the list, you can contact us.

TryptophanEthanolamineL-ThreonineL-Glutamic AcidL-Cystathionine
Valineβ-AlanineCysteineL-MethionineL-Carnosine
LeucineL-AlanineTaurineL-HistidineL-Anserine
IsoleucineL-SarcosineHydroxy-L-prolineL-α -Aminoadipic AcidL-Cystine
Phenylalaniney-Amino-n-butyric AcidL-Isoleucine5-HydroxylysineL-Homocystine
TyrosineD, L-B-Aminoisobutyric AcidL-LeucineL-PhenylalanineL-Glutamine
GlycineL-SerineAsparagine1-Methyl-L-histidine6-Aminocaproic acid
CysteineCreatinineL-OrnithineL-Arginine5-hydroxytryptamine
MethionineL-ProlineL-Aspartic AcidL-Citrulline5-Hydroxytryptophan
ArginineL-Valine L-LysineL-Tyrosine 

Advantages of Amino Acid Analysis Service

  • Advanced platform: Ultra-high resolution, ultra-high sensitivity and fast scanning speed QE HF mass spectrometry platform is used for detection, with large throughput and more compound information can be identified. Multiple reaction monitoring (MRM) technology is used to achieve precise qualitative and absolute quantitative.
  • High-quality database: The primary and secondary mass spectra in the database are all real spectra detected by the high-quality, high-resolution, high-resolution Orbitrap mass spectrometer using standard products. The compound identification accuracy is high.
  • Strict quality control: isotope internal standard calibration, external standard method quantification. Manual proofreading of standards. Standard R2>0.99.
  • Personalized service: Have a professional information analysis team to analyze the data results. Personalized information analysis can also be carried out according to customer needs.

Sample Requirements

  • Serum and Plasma ≥ 100 µL
  • Tissue ≥ 50 mg
  • Fresh stool ≥ 100 mg
  • Cell ≥ 1*107

Duplicate samples:

  • Animal samples (various tissues, plasma) ≥10 replicates per group
  • Microbial samples ≥ 6 replicates per group
  • Cell sample ≥ 3 replicates per group
  • Clinical samples (serum, urine, various tissues, etc.) ≥30 cases in each group

Data Analysis

  • Raw data
  • Data preprocessing
  • Data quality control
  • Metabolite annotation

Reference

1. Hirasawa, Takashi, and Hiroshi Shimizu. "Recent advances in amino acid production by microbial cells." Current opinion in biotechnology 42 (2016): 133-146.

*For Research Use Only. Not for use in diagnostic procedures.

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