The gut microbiota residing in the intestinal tract composes of an abundant and diverse population of microbes including bacteria, viruses, and microbial eukaryotes, which plays a significant role in maintaining host health. It bidirectionally affecting physiological processes through the "cross-talk" with the host cells, these various processes include immune modulation, neurotransmitter regulation, hormones secretion and regulation, and the production of many antioxidants and metabolites. The composition of the gut microbiota varies from individual to individual, but the microbial community in each individual is relatively stable over time. The overuse of antibiotics poses unignorable issues toward the homeostasis of the gut ecosystem. Because they not only act on microbes that cause infections but also affect the "good microbes" and even cause dysbiosis. Antibiotic resistance testing can be a valuable tool in studying the antibiotic-microbiota-health topics.
Antibiotic resistance of microbes is encoded in their resistance genes, either intrinsic or acquired, which indicates that the antibiotic susceptibility can be interpreted through high-throughput sequencing data. While the phenotype is determined by many factors, the antibiotic resistance testing, antibiotic sensitivity testing, or antibiotic susceptibility testing (AST) can be a rapid, direct, and effective approach to evaluate the sensitivity that a specific microorganism can be affected by antibiotics. Antibiotic resistance testing has significance in studying microbiota and host homeostasis.
Figure 1. Examples of antibiotic susceptibility testing methods (left: E-tests; right: disk diffusion method).
Creative Proteomics, a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art instrumentations, provides holistic solutions for your gut microbiota research. Our comprehensive antibiotic resistance testing services cover the most widely used methods, including dilution methods (broth and agar), disk-diffusion method, E-test, automated methods, mechanism-specific tests, and microfluidic-based optical and electrochemical AST, which will assist you in unraveling the complex factors that shape commensal gut microbiota susceptibility and resilience to antibiotics. Our testing service offers you a one-stop solution from experiment design to result delivery. The protocols include testing of the most commonly used antibiotics:
Creative Proteomics is one of the most trusted companies in providing gut microbiota research services. We strive to provide the best strategies for your gut microbiota and related studies, and we will offer the most suitable strategies according to your sample and research purpose. To find out more about our antibiotic resistance testing services, please feel free to contact us.
1. Moskowitz JE, Devkota S. Determinants of Microbial Antibiotic Susceptibility: The Commensal Gut Microbiota Perspective. Cell Host & Microbe. 2019;26(5):574-6.
2. Khan ZA, Siddiqui MF, Park S. Current and Emerging Methods of Antibiotic Susceptibility Testing. Diagnostics (Basel). 2019;9(2):49.
3. Frieri M, Kumar K, Boutin A. Antibiotic resistance. Journal of infection and public health. 2017;10(4):369-78.
4. Hu Y, Yang X, Qin J, et al. Metagenome-wide analysis of antibiotic resistance genes in a large cohort of human gut microbiota. Nature communications. 2013;4(1):1-7.
*For Research Use Only. Not for use in diagnostic procedures.
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