It has been well elucidated that the enteric microbial communities that inhabit the human gastrointestinal track exert a multitude of functions and broadly impact human health. Gut microbiota is widely studied by multi-omics including (meta)genomics, (meta)transcriptomics, (meta)proteomics and metabolomics, among which metabolomics studies have been increasingly used in gut microbiota to perform the large-scale metabolic profiling and characterize small molecules produced by gut microbes.
As intestinal epidermal cells reacting to gut microbes or their metabolites and produce mediators that concern with physiological activities, metabolomics is often considered the most direct angle to study the relationship between gut microbiota and the physiological state, fundamental understanding of disease, and the search for potential biomarkers, whereby identification of unknown metabolites, mapping unique metabolome fingerprints of specific cellular processes, and determine specific pathways. Nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and Fourier transform infrared (FTIR) spectroscopy are main technologies that are applied in gut metabolomics.
Figure 1. Interaction between gut microbiota and host whereby microbial metabolites (Moya 2016)
Creative Proteomics, as a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art techniques as well as well-experienced scientists, provides one-stop metabolomics services for your gut microbiota research. The typical workflow falls into the following steps:
Sample handling is a key step in metabolomics research, with the quality of the sample weighing heavily on the subsequent steps. We provide delicate metabolite extraction techniques according to the state of the raw material and research purpose, to guarantee an outcome with high fidelity and reliability. The methods that we apply to treat the samples generally include liquid-liquid extraction, solid phase extraction and filtration.
Separation achieves various goals: to increase resolution and reduce ion suppression in the identification, and the retention time of the analyte serves as valuable information. We provide the following techniques for metabolite separation:
◆ Liquid chromatography (LC)
◆ High performance liquid chromatography (HPLC)
◆ Gas chromatography (GC)
◆ Capillary electrophoresis (CE)
The identification brings comprehensives insights into metabolomic study – with our advanced lab equipment, we run tests to identify assays including but not limit to lipids, bile acids, stratum corneum, metal ions, and amino acids. The following techniques are at your disposal:
◆ Mass spectrometry (MS)
◆ Nuclear magnetic resonance (NMR) spectroscopy
◆ Fourier transform infrared (FTIR) spectroscopy
◆ Raman spectroscopy
Coupled with powerful analysis platforms, we provide targeted metabolomic profiling, untargeted metabolomic profiling, accurate absolute quantification, and even further custom statistical analyses.
With adequate analytical tools and pipelines, we promise to provide the best solutions to meet your requirements. If there is any need of yours, do not hesitate to contact us!
1. Moya A, Ferrer M; Functional Redundancy-Induced Stability of Gut Microbiota Subjected to Disturbance. Trends in Microbiology. 2016
For Research Use Only. Not for use in diagnostic procedures.