Gut Microbiota Metaproteomic Services


Gut Microbiota Metaproteomic Services

With ever-increasing research into the genomics, we started to realize that merely by exploitation of genetic information is not enough to unveil the laws of life activity, which makes it significant to get a comprehensive understanding of proteins, the principal embodiment of life activities - These proteins are produced by transcription and translation of genes, which may be expressed variably in different environments even in the same organism.

Metaproteomics refers to the study of all protein collection recovered from the microbial community, which provides informative details concerning functional aspects of the microbiota. By profiling gut metaproteomics, we can get the panorama of the connection between gut microbial diversity, functions and the impact on host biology. Metaproteomics has been widely applied to study gut microbiota varied from factors such as age diet and has also thrown light upon research into dysbiosis.

Creative Proteomics, as a premier corporation that has many years of experience in proteomics, presents state-of-the-art techniques to gut metaproteomics analysis. The service workflow generally contains 3 steps: protein extraction, protein separation, and identification & analysis.

Gut Metaproteomics Analysis

Protein Extraction

Sample preparation (protein extraction) is a key step in proteomics research, with the quality of the sample weighing heavily on the subsequent steps. We provide delicate protein extraction techniques according to the state of the raw material and research purpose, to guarantee an outcome with high fidelity and reliability.

Protein Separation

The separation of proteins in metaproteomics is consistent with the study of general proteomics, with the most commonly used methods of two-dimensional electrophoresis and chromatography (such as HPLC). Two-dimensional electrophoresis presents the changes of specific proteins in the whole metaproteomics in a visual form, and the protein reference map can be constructed for the subsequent analysis of the comparative proteome and functional proteome. While chromatographic methods are more effective and much easier to operate, though the resolution is not as eminent as two-dimensional electrophoresis.

Identification & Analysis

Proteins can be identified by electrophoresis combined with mass spectrometry (MS) analysis, while it is more common and more efficient to use multiple chromatographic separation with MS for metaproteomics analysis. Available study methods are as follows:

  • ◆ Mass spectrometer, Tandem mass spectrometry (MS/MS)
  • ◆ Matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF/MS)
  • ◆ Electrospray ionization mass spectrometry (ESI/MS)
  • ◆ Quadrupole Time-of-Flight Mass Spectrometry (QTOF-MS)
  • ◆ Microfluidic-based tools
  • ◆ Isotope affinity tags

Combined advanced technology with the rich metaproteomics database of our platform, we provide superior gut metaproteomics analysis solutions. If there is any need of yours, do not hesitate to contact us!

For Research Use Only. Not for use in diagnostic procedures.

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