Isolation and Cultivation of Intestinal Microbes

gutfloraomics

Isolation and Cultivation of Intestinal Microbes

Isolation and Cultivation of Intestinal Microbes

The gastrointestinal tract harbors trillions of commensal microorganisms, collectively known as the microbiota, which has a tremendous impact on the health of the host by regulating homeostasis within the gut, though, among which most are anaerobes and a large number of them are considered 'unculturable' for having never been cultured outside of the hosts. Although the powerful culture-independent technologies that bloomed and progressed enormously in the recent decades have enabled us to gain insight into a deep and intrinsic level, the culture-based approaches are still required to understand microbe characteristic features and phenotypes, to uncover their nutritional preferences and diversified biosynthetic capabilities, and even facilitate the pathological studies.

Creative Proteomics is a preeminent corporation that specializes in multi-omics. And additionally, we also provide versatile solutions involving other practical technologies to assist with your gut microbiota research. Equipped with cutting-edge lab equipment and served by well-experienced scientists, we have extensive experience in providing abundant solutions in isolation and cultivation of intestinal microbes.

Traditional Isolation and Cultivation

Isolation and cultivation are the classical foundation of associating phenotypes to specific strains or elucidating physiological interactions, which is still an irreplaceable position in microbial research. Our labs are equipped with various incubators that are available for all kinds of aerobes and anaerobes, and we have adequate experiment ground that allows for separate culture rooms for fungi, and viruses to avoid cross-contamination. We are your qualified trustee for microbe isolation and cultivation.

Culture Medium

Commercially media from the world's top manufacturers including CHROMagarTM and BDTM are available in our labs, as well as custom and well-established in-house media. With suitable media and other conditions, a number of intestinal microbes hitherto considered unculturable are potentially culturable. Here are some of our most used media.

Sheep Blood agar M58BL agar
CLED mediumBrucella HK agar
Mannitol salt agarBBE agar
EF agarKanamycin CW agar
DHL agarCCFA agar
Gut microbiota mediumLBS modified agar
Listeria enrichment brothGifu anaerobic medium agar

High-throughput Isolation and Sorting

Numerous cultivation strategies have been developed to increase the throughput in isolating microbes, which could reduce biases of traditional cultivation strategies, and address the issues such as labor-intensive and low-throughput. We offer multiple schemes to meet your needs in isolation and cultivation of intestinal microbes of large amounts. Microfluidics rapidly emerged as one of the key techniques that open up new experimental possibilities in microbiology. The microfluidic platform isolates and cultivates microbial cells in millions of picoliter droplets and automatically sorts droplets based on colony density. We provide microfluidic technologies for large scale screening. As the most up-to-date and cutting-edge technology that we utilize in high-throughput isolation and sorting, microfluidic screening compensates for the shortcomings of traditional methods.

One Step Further

With advanced experimental facilities and resources, we offer various solutions based on the isolation and cultivation, which include but not limited to:
◆ Personalized culture collections
◆ Culturomics
◆ Cultivation-based multiplex phenotyping

Creative Proteomics dedicates to providing the highest level of solutions in gut microbiota research. We will offer the most suitable strategies according to your sample and research purpose. Our specialty covers the full range of multi-omics, coupled with other practical tools and resources, we provide the most comprehensive gut microbiota solutions you will ever get. Leave the laboring to us, and we will provide the isolation and cultivation services which will tremendously facilitate your gut microbiota studies. To find out more solutions for intestinal microbe identification and cultivation solutions, please feel free to contact us.

For Research Use Only. Not for use in diagnostic procedures.

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