The gastrointestinal tract is host to an abundant and diverse population of microbes including bacteria, viruses and microbial eukaryotes, which serve various physiological functions that are essential to host homeostasis including metabolism and immune function. Thus, many compare the gut microbiota to a hidden 'organ', and this 'organ' interacts with other organs or systems directly or indirectly, where signs can be traced by multi-omics technologies with regard to the host responses to the crosstalk between the host and the microbiota.
Although classical detecting methods, such as western blotting, ELISA, and quantitative PCR, have brought to the primal understanding of gut microbiota and host interaction, these technologies are inherently low-throughput and narrow in scope. While the advances of multi-omics technologies, especially next-generation sequencing, transcriptional assays, chromatography, and mass spectrometry, have shed light upon correlational studies between the host and the microbiota, and provide enormous convenience of monitoring host responses to the gut microbiota, which facilitate the discovery of candidate molecules and pathways that underlie mechanisms.
Figure 1. The Gut Microbiota Affects Host Pathophysiology as an Endocrine Organ (Busnelli 2020)
Creative Proteomics is a preeminent corporation that specializes in multi-omics. Equipped with cutting-edge techniques and served by well-experienced scientists, we have extensive experience to assist you in monitoring host responses and provide abundant research solutions into every aspect from the fundamental mechanism studies to clinical application, and leading to decisive breakthroughs.
Host responses to the dense and dynamic gut microbiota are often mediated by the secretion of proteins and other metabolites into the gastrointestinal tract. In order to gain a comprehensive view of microbiota-host interaction and monitoring host responses, we provide technical routes not only for traditional metaproteomics that focused on microbiota-derived targets but host-centric proteomics which differs from the former mainly in sample fraction, fractionation method, and search database, both covering information including diversity, variability, and dynamic range profiling. We offer host responses monitoring solutions mainly from metatranscriptomics, metaproteomics, and metabolites.
● We provide services widely covering the issue not only including mRNAs that indicate microbial activities to the utmost but non-protein coding genes and non-coding RNAs that also play instrumental roles in the system, to ensure the comprehensive understanding of the transcriptional products.
● The large-scale transcriptional assays of feces, intestinal tissue, and other samples provide a highly sensitive analysis of microbiota-induces host responses.
● We provide gut metaproteomics solutions for gaining the panorama of the connection between gut microbial diversity, functions and the impact on host biology. Mass spectrometry-based proteomics would greatly serve this purpose of monitoring host protein secretion into the gastrointestinal tract, feces, and other proper samples.
● Our solutions fully cover the entire workflow of protein extraction, protein separation, and identification & analysis.
● Metabolomics has shown great promise in the characterization of host-microbiota interactions. Both microbiota-derived and host-secreted metabolites will work as critical indicators for diseases or treatment monitoring.
● We provide targeted metabolomic profiling, untargeted metabolomic profiling, accurate absolute quantification, and custom statistical analyses to meet your needs in host response monitoring.
Creative Proteomics dedicates to providing the highest level of solutions in gut microbiota research. We will offer the most suitable strategies according to your sample and research purpose. Our specialty covers the full range of multi-omics including metagenomics, metatranscriptomics, metaproteomics, and metabolomics, coupled with powerful bioinformatics platforms, and other practical tools and resources, we provide the most comprehensive gut microbiota research solutions you will ever get. To find out more solutions for gut microbiota related host response monitoring, please feel free to contact us.
1. Busnelli M; et al. The Gut Microbiota Affects Host Pathophysiology as an Endocrine Organ: A Focus on Cardiovascular Disease. Nutrients. 2020, 12(1), 79.
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