Creative Proteomics is a preeminent corporation that specializes in multi-omics. Equipped with state-of-the-art techniques and served by well-experienced scientists, Creative Proteomics provides one-stop services for your gut microbiota research. Thus, in addition to multi-omics analysis services, we also offer you comprehensive and practical tools to facilitate your research.
Real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a sensitive, powerful, and low-cost method used to amplify and simultaneously monitor, and quantify or semi-quantify targeted DNA molecules, which is a conventional technology in biology labs to provide insights into gene expression and phenotypes of microorganisms on the genetic level. Studies of the microbial communities by qPCR methods have revealed striking similarities as well as differences in the ratio of certain species in specific groups of people that of different ages, diet habits, and health conditions. There are two detection methods of qPCR, the first is based on sequence-specific probe served as fluorescent signal report molecules; the second is based on generic non-sequence-specific double-stranded DNA-binding dye such as SYBR green. And both methods are in need of delicately designed primers.
Figure 1. The principles of qPCR using TaqMan chemistry (Schaad 2003)
Creative Proteomics provides qPCR probes and primers in highly optional quenchers and fluorescent dyes ranging from the visible spectrum up to the near infrared range. These probes and primers are synthesized at different scales using natural DNA bases or RNA analogs such as Bridged Nucleic Acid (BNA) to improve technologies that require high-affinity or high-specificity probes, such as SNP detection, expression profiles, and in situ hybridization. We offer cost-effective, stable, and ready-to-use synthesized qPCR probes not only for conventional qPCR but advanced techniques such as multiplex assays that allow detection of multiple targets within the same PCR reaction. We provide solutions in major probe formats as follows:
◆ Hydrolysis (TaqMan) probes
◆ Molecular beacons
◆ Eclipse probes
◆ Scorpions Probes
These qPCR probes and primers can be applied in versatile research areas including but not limited to:
◆ Gene expression analysis
◆ Non-coding RNA analysis
◆ Variation analysis
◆ DNA quantitation
◆ Pathway analysis
Creative proteomics is one of the most trusted companies in the field, we strive to provide the best strategies for your gut microbiota and related studies, and we will offer the most suitable strategies according to your sample and research purpose. With wide-range options of quenchers and fluorescent dyes, and fine purification and quality control by state-of-the-art techniques, we provide fast and reliable qPCR probe and primer synthesis service to meet your needs in multiple research areas. For additional information on our real-time PCR probes and primers synthesis services or to get price inquiry, please feel free to contact us.
1. Schaad S; et al. Advances in Molecular-Based Diagnostics in Meeting Crop Biosecurity and Phytosanitary Issues. Annual review of phytopathology. 2003, 41: 305-24.
For Research Use Only. Not for use in diagnostic procedures.