The human gastrointestinal tract is colonized by a vast array of microbes, which form communities of bacteria, viruses and microbial eukaryotes. Gut microbiota plays a pivotal role in human health and fitness by producing nutrients and by dominating gut over potential pathogenic microorganisms, and even by influencing the immune system. Real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is used to amplify and simultaneously monitor, and quantify or semi-quantify targeted DNA molecules, which is a conventional technology in biology labs to provide insights into gene expression and phenotypes of microorganisms on the genetic level.
qPCR assay is a practical, low-cost method for monitoring the variations of microbes of the gut. Studies of the microbial communities by qPCR methods have revealed striking similarities as well as differences in the ratio of certain species in specific groups of people that of different ages, diet habits, and health conditions. For instance, Firmicutes and Bacteroidetes, the two dominant bacterial divisions of gut microbiota in mammals, are closely related to body fat in humans and some other mammals.
Creative Proteomics assists researchers in developing customized real-time PCR experiments, and provides rapid and reliable real-time PCR services, which can be used for quantification of gene expression (relative quantification and absolute quantification), miRNA expression, genotyping, and copy number determination. The one-stop service workflow covers RNA or DNA extraction, qPCR primer design and validation, qPCR reaction optimization, and complete data analysis.
◆ Total RNA
◆ Total DNA
◆ Plasmid DNA
◆ Cell culture
◆ TaqMan probe and SYBR Green dye both available
◆ Absolute quantification and semi-quantification both available
◆ Custom assay design
◆ Custom primers/probes design
◆ Exquisite sample preparation
◆ High sensitivity and reproducibility
◆ Comprehensive data analysis and interpretation
◆ Data visualization
We dedicate to providing the highest level of service in gut microbiota research. Our custom analysis will provide valid solutions and guarantee a high-quality report for your research. We will arrange experienced scientists to provide you with the professional advice of qPCR applied in gut microbiota research and optional services according to your project. To find more about our real-time PCR services, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.