The gastrointestinal tract harbors trillions of commensal microorganisms, collectively known as the microbiota, which has a tremendous impact on the health of the host by regulating homeostasis within the gut. Any alteration in the composition microbiota may lead to disorders that include inflammatory bowel disease, diabetes, allergies, mental disorders, and cancer.
Figure 1. Mechanism of RNAi-mediated silencing (Singh 2016)
RNA interference (RNAi) is an epigenomic process in which genes are silenced post-transcriptionally by certain RNA molecules (such as miRNAs, and siRNAs) neutralizing targeted mRNA molecules or directing specific epigenetic modifiers. These RNAs introduced into cells can selectively and robustly induce suppression and sometimes upregulation of specific genes of interest, which makes RNAi a valuable research tool both in cell culture and in living organisms, and can help to identify the components necessary for a particular cellular process or an event. In addition to that, RNAi is a vital part of the immune response to viruses and other foreign genetic material, and various gut probiotic strains are also effective in modulating immune responses via RNAi.
Medical & Clinical Applications
Creative Proteomics, a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art techniques as well as well-experienced scientists, provides one-stop RNAi services for your gut microbiota research, to meet multiple needs of basic research and medical applications. We utilize RNAi technologies to achieve robust genetic screening, regulation, and functional interpretation, and to help you understand complex gut microbial systems and improve human health. We offer custom RNAi services as follows:
◆ Custom RNAi (miRNA or siRNA or shRNA) design & synthesis & modification
◆ Large-scale screening
◆ Gene knockdown
◆ RNAi transfection service
◆ Inhibitory effect testing service
◆in vivo application development
RNAi induced gene silencing can be practiced in multiple model organisms, including human, RNAi can also be used in the determination of gene function, and fast progress in RNAi technology has shown promise for use in treating viral infections and cancer. We dedicate to providing the highest level of service in gut microbiota research. We will offer the most suitable strategies according to your sample and research purpose. To find out more about our RNAi services, please feel free to contact us.
1. Singh D; et al. RNA Interference Technology – Applications and Limitations. RNA Interference. 2016
For Research Use Only. Not for use in diagnostic procedures.