The microbial communities colonized in the human gastrointestinal tract have a significant impact on human health and fitness. Gut metaproteomic studies of whole microbial communities provide insights into the phenotypes of microorganisms on the protein level, and we owe the rapidly increased progress to many technological advances in chromatography and mass spectrometry. It is widely known that in proteomic or metaproteomic research, the quality of sample preparation often determines the result of subsequent research. As complex as the sample is, additionally without PCR methods for amplification as nucleic acid research has, reliable and quantitative metaproteomic analysis is a tremendously challenging job, which makes a proper and efficient first step of sample handling undoubtedly critical.
Figure 1. An overview of metaproteomic analysis workflow (Petriz 2017)
Creative Proteomics, as a preeminent biotech corporation that has many years of experience in proteomics, provides skillful sample treatment for your gut metaproteomic samples. We have full confidence in dealing with key points such as protein concentration, purification, and excluding the impact of highly abundant components.
The sample preparation strategy needs to be adjusted according to the target protein and subsequent protein analysis methods. We dedicate to providing the highest level of service in gut microbiota research. To find out more about the optimum custom strategy for your research, please feel free to contact us.
1. Petriz BA, Franco OL. Metaproteomics as a Complementary Approach to Gut Microbiota in Health and Disease. Frontiers in Chemistry. 2017, 5:4.
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