The microbial communities colonized in the human gastrointestinal tract have a significant impact on human health and fitness. Gut metaproteomic studies of whole microbial communities provide insights into the phenotypes of microorganisms on the protein level, and we owe the rapidly increased progress to many technological advances in chromatography and mass spectrometry. It is widely known that in proteomic or metaproteomic research, the quality of sample preparation often determines the result of subsequent research. As complex as the sample is, additionally without PCR methods for amplification as nucleic acid research has, reliable and quantitative metaproteomic analysis is a tremendously challenging job, which makes a proper and efficient first step of sample handling undoubtedly critical.
Figure 1. An overview of metaproteomic analysis workflow (Petriz 2017)
Creative Proteomics, as a preeminent biotech corporation that has many years of experience in proteomics, provides skillful sample treatment for your gut metaproteomic samples. We have full confidence in dealing with key points such as protein concentration, purification, and excluding the impact of highly abundant components.
We provide the following sample preparation services:
- Sample pretreatment: Removal of possible coextracted contaminants is also a fair sample dependent consideration that might influence the results. Necessary measures are taken to deal with your sample to ensure safe downstream operations.
- Protein extraction: A wide range of protocols for efficient extraction of proteins from microbial cells is available, allowing for optimal extraction from a variety of samples. A generalized extraction protocol includes cell lysis (physical or chemical), solubilization and chemical reduction of the metaproteome, separation of the metaproteome from residual sample components, and precipitation of the metaproteome.
- Proteolysis: Once a sufficient amount of proteins has been extracted, precipitated, and concentrated, use trypsin to achieve extensive digestion.
- Cleanup: Clean up the digested sample with RP solid-phase extraction (SPE) cartridge. Alternatively, sample digestion and cleanup can be performed using a filter-aided sample preparation protocol.
Advantage of our protein sample preparation services:
- Cutting-edge platform with ultrafiltration technology
- Experienced scientists dealing with various samples
- Optimized and quality control workflow to work with various samples
- Project design based on the properties of target proteins
The sample preparation strategy needs to be adjusted according to the target protein and subsequent protein analysis methods. We dedicate to providing the highest level of service in gut microbiota research. To find out more about the optimum custom strategy for your research, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.
