The gut microbiota has a non-negligible influence on nutrient acquisition, homeostasis stabilization, immune responses, and pathogen colonization and eradication. Small interfering RNAs (siRNAs) are a class of double-stranded RNA molecules of 20-25 base pairs. siRNAs are active in the RNA interference (RNAi) pathway by interfering with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, thus preventing translation. As the discovery of RNA interference and whose potential of disease treatment, the interest in siRNA working as a new approach of therapy is rising. siRNA offers opportunities to precisely target genes that contribute to gastrointestinal (GI) pathologies, which might be affected by external environmental factors and intestinal microbiota as well.
Creative Proteomics provides genome-wide siRNA sequencing and siRNA microarray, and siRNA design services, to shed light on your research into siRNA, and more hopefully, that associated with gut microbiota and/or health, maybe even potential solutions to address related issues.
We provide siRNA profiling service by using next-generation sequencing or massively parallel high-throughput DNA sequencing, allows for the examination of tissue-specific expression patterns, disease associations, or isoforms of siRNAs, and to discover previously uncharacterized siRNAs. With advanced technology and experienced experts, we possess the full ability to:
◆ Obtain siRNA abundance levels from sequence reads
◆ Discover novel siRNAs
◆ Determine the differentially expressed siRNA
◆ Determine the siRNA associated mRNA gene targets
Microarray is a powerful high-throughput tool that is capable of conducting a genome-wide analysis of siRNA mechanisms, detecting multiple samples in parallel and monitoring the expression of thousands of small noncoding RNAs in a single run. We provide one-stop service of siRNA microarray analysis, the major protocol includes siRNA oligo probe design, array fabrication, and siRNA target preparation, target-probe hybridization on array, signal detection, and data analysis. The process can be standardized and allows the reproducible profiling of interaction between siRNA and gut microbiota.
Synthetic siRNA can also be introduced into the cell and result in the knockdown or silencing of target genes. With advanced technologies, state-of-the-art equipment, and experienced scientists, we are able to offer one-stop custom siRNA services for your research – from siRNA design to in vitro and in vivo expression, especially for projects across human, mouse and rat genomes. Our high-throughput screening and delivery system together with the powerful bioinformatics platform will be more than helpful to your projects!
Creative Proteomics' goal is to deliver efficient and professional solutions for your research project. To find out more about the siRNA services, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.