Metabolome can be described as the complete set of metabolites (molecules with low molecular weight) present within an organism, cell, or tissue. The analysis of gut microbial metabolites will facilitate the understanding of gut physiology and the role of gut microbiota in the gastrointestinal tract. Metabolomics is a promising field for investigating the molecular mechanism of gut microbiota, the general approaches of measuring these molecules are divided into two groups: targeted metabolomics and untargeted metabolomics. Targeted metabolomics is a quantitative analysis of known metabolites, which helps in the overall understanding of the massive array of metabolites, their kinetics, as well as their pathways. Integrating data from other omics studies, targeted metabolomics will help to elucidate functions and impacts that the gut microbial communities have on human health, and also provide a novel horizon to monitor disease state or in physiological stimuli.
Creative Proteomics, as a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art techniques as well as well-experienced scientists, provides one-stop targeted metabolomics services for your gut microbiota research. The typical workflow falls into the following steps:
Sample handling is a key step in metabolomics research, with the quality of the sample weighing heavily on the subsequent steps. The analysis of metabolome covers a multitude of categories including but not limited to bile acids, indoles, fatty acids, short-chain fatty acids, and vitamins, which have distinct physical and chemical properties. Thus, sample pre-treatment methods should base on and vary flexibly according to the targeted metabolites of interests. We provide sample handling services customized to the relative abundance and physicochemical attributes of a metabolite subset that is to be analyzed.
We offer metabolites data acquisition assays with optional solutions that involve coupling chromatography techniques with spectrometry techniques. The following methods are at your disposal:
◆ Liquid chromatography-mass spectrometry (LC-MS)
◆ Gas chromatography-mass spectrometry (GC-MS)
◆ Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
◆ Flow injection-tandem mass spectrometry (FI-MS/MS)
Coupled with powerful analysis platforms, we provide not only metabolites quantitation services, but also the corresponding kinetics analysis, pathways analysis, and other systematic bioinformatics analyses.
With adequate analytical tools and pipelines, we dedicate to providing targeted metabolomics profiling service with excellent analytical precision, unequivocal identification, and custom analyses. If there is any need of yours, do not hesitate to contact us!
For Research Use Only. Not for use in diagnostic procedures.