Metabolome can be described as the complete set of metabolites (molecules with low molecular weight) present within an organism, cell, or tissue. The analysis of gut microbial metabolites will facilitate the understanding of gut physiology and the role of gut microbiota in the gastrointestinal tract. Metabolomics is a promising field for investigating the molecular mechanism of gut microbiota, the general approaches of measuring these molecules are divided into two groups: targeted metabolomics and untargeted metabolomics. Untargeted metabolomics aims to study both known and unknown metabolites, seeking to detect as many distinct features as possible in a single analysis. Integrating data from other omics studies, untargeted metabolomics could be a valid approach for a multitude of issues such as the assessment of health-influencing factors, and novel biomarker discovery.
Creative Proteomics, as a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art techniques as well as well-experienced scientists, provides one-stop untargeted metabolomics analysis services for your gut microbiota research. The typical workflow falls into the following steps:
Sample handling is a key step in metabolomics research, with the quality of the sample weighing heavily on the subsequent steps. The sample extraction of untargeted metabolomics should cover the range of metabolites as wide as possible. We provide delicate untargeted metabolites extraction techniques according to the state of the raw material and research purpose, going through all the processes from tissue homogenization and cell lysis to lipid extraction in a custom solution, to ensure the following steps run smoothly.
Separation achieves various goals: to increase resolution and reduce ion suppression in the identification, and the retention time of the analyte serves as valuable information. We provide the following techniques for metabolites separation:
◆ Gas chromatography (GC)
◆ Liquid chromatography (LC)
◆ High-performance liquid chromatography (HPLC)
◆ Ultra-high-performance liquid chromatography (UHPLC)
The identification brings massive data for untargeted metabolomics study, the following techniques are at your disposal:
◆ Mass spectrometry (MS)
◆ Tandem mass spectrometry (MS/MS)
◆ Nuclear magnetic resonance (NMR) spectroscopy
◆ Quadrupole Time-of-Flight (Q-TOF) Mass Spectrometers
Whereby using methods such as univariate analysis, multivariate, and multiway, and along with comprehensive commercial databases, we provide untargeted metabolomics data analyses including building classification models to describe the data under observation, and running pathway analysis or correlation-based network analysis biological interpretation.
With adequate analytical tools and pipelines, we dedicate to providing untargeted metabolomics analysis solutions with broad coverage to meet your requirements. If there is any need of yours, do not hesitate to contact us!
For Research Use Only. Not for use in diagnostic procedures.