The human gastrointestinal tract harbors a diverse and dynamic microbial community that is dominated by bacteria, viruses, archaea, fungi and other eukaryotes. which directly or indirectly impacts human physiological activity and health. Understanding gut microbiome functions frequently requires to have microbes sequenced for experimental validation and reference genome sequences to interpret metagenome datasets and guide functional analysis. Sequencing the genome of a species with existed reference genome is known as resequencing. Discovering what makes the new genome different from the reference is much more convenient than assembling a novel genome. Thus, the improved resource of gastrointestinal microbe reference sequences evades de novo assembly of metagenomes to the utmost extent and enables accurate and cost-effective analysis of human gastrointestinal microbiota.
The next-generation sequencing technology coupled with the increasing volume of genome sequences offers strategies for more effective genetic mapping and genome analysis, whole-genome resequencing (WGRS or WGR) has become a rapid and effective method to study mutation, species origin, development, growth, etc., which can be achieved whereby sequencing the entire genome of specific individuals, comparing the sequence to that of a known reference genome, and bioinformatic analysis such as identification of genetic variations and discovery of functional genes and markers of important traits or even other genetic changes of the sequenced species, which could provide an extremely valuable insight into the gut microbiota research.
Creative Proteomics utilizes state-of-the-art platforms for whole-genome resequencing, multiple software for bioinformatics analysis, and abundant database for comparison and annotation, so we can provide you with the high level of expertise required for your specific project in a comprehensive manner. We are fully qualified to provide multiple resequencing and related services including but not limit to genetic variation detection, genetic map construction, mapping loci associated with specific traits and evolutionary genetics research. The workflow generally falls into the following steps:
We strive for the best customer experience and provide services at fast turnaround, high-quality data, and affordable prices. Our custom analysis not only guarantees the high quality of sequencing results but also can provide more sparkling ideas for your research. To find more about whole-genome resequencing for your gut microbiota research, please feel free to contact us.
For Research Use Only. Not for use in diagnostic procedures.